23. A nucleic acid vector for use in in vivo delivery of a nucleic acid of interest right into a focused receiver bacterial mobile, claimed nucleic acid of curiosity developing a presented effect on said qualified receiver bacterial mobile, whereby claimed vector comprises: explained nucleic acid of interest, and
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Since stated conditional origin of replication 查看詳細說明 is inactive during the specific receiver bacterial cell due to the absence of reported offered protein, peptid, RNA, nucleic acid, molecule or any mixture thereof in reported receiver bacterial cell, claimed conditional origin of replication can be chosen according to the certain receiver bacterial mobile to get focused.
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comprising claimed nucleic acid of desire, whereby said vector more comprises a conditional origin of replication that is inactive in the specific receiver bacterial mobile but is Energetic in a donor bacterial cell, and reported vector is devoid of antibiotic resistance marker,
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30. a way for ex vivo modulating a microbiome from an natural environment by accumulating specific receiver bacterial mobile from reported ecosystem and by offering a nucleic acid of interest right into a qualified receiver bacterial mobile of mentioned microbiome, stated nucleic acid of desire generating a specified impact on said targeted receiver bacterial cell, wherein claimed process comprises getting in contact with a nucleic acid vector comprising stated nucleic acid of curiosity with claimed microbiome,
In a specific embodiment, stated offered influence is making the receiver bacterial cell stop producing a given molecule.
wherein said vector is a phagemid or recombinant phage nucleic acid vector, said vector comprising a conditional origin of replication that's inactive during the focused receiver bacterial mobile but is active inside of a donor bacterial cell, whereby reported conditional origin of replication is SEQ ID NO: four which is the origin of replication in the phage-inducible chromosomal island (PICI) with the Escherichia coli pressure CFT073, or said origin of replication is modified to be the sequence of SEQ ID NO: 6 or SEQ ID NO: 7, and whereby explained conditional origin of replication is Lively in said donor bacterial mobile because mentioned donor bacterial mobile expresses a primase-helicase comprising SEQ ID NO: 8 and whereby reported vector is devoid of antibiotic resistance marker.
In summary, PICI primase and origin can be stably taken care of in creation strains, are appropriate with lambda-centered phagemids packaging judging because of the titers obtained and the payloads are dependent on the existence of its cognate primase for Energetic replication and upkeep in target strains.
As used herein, the expression “conjugative plasmid” refers to your plasmid that may be transferred from a person bacterial cell to a different for the duration of conjugation and a “donor bacterium”, as used herein, is then a bacterium that's able to transferring a conjugative plasmid to another bacterium.
this sort of administration sorts could be solid, semi-reliable or liquid, with regards to the method and route of administration. such as, formulations for oral administration may be delivered by having an enteric coating that allows the vector, bacterial supply car or truck or donor bacterial mobile, in the formulation to resist the gastric ecosystem and go in to the intestines. much more commonly, vector formulations, bacterial shipping and delivery motor vehicle formulations or donor bacterial mobile formulations for oral administration could be suitably formulated for supply into any sought after Portion of the gastrointestinal tract. Moreover, appropriate suppositories may be useful for shipping and delivery into your gastrointestinal tract. Various pharmaceutically or cosmetically appropriate carriers, diluents and excipients practical in pharmaceutical or veterinary or beauty compositions are recognised for the skilled person
本发明涉及用于调节宿主微生物组的感兴趣的核酸，涉及编码所述核酸的载体以及涉及用于通过递送所述感兴趣的核酸来调节宿主微生物组的方法。